When more than one condition is somewhat adverse to microbial growth, their inhibitory effects are cumulative. Bacterial growth curve and its significance. When a broth culture is inoculated with a small bacterial inoculum, the population size of the bacteria increases showing a classical pattern. Microbial growth on meat products, as well as other foods, is affected, not only by the type and level of initial contamination but also by various factors associated with the product (intrinsic) or its environment (extrinsic). Factors that Influence Microbial Growth December 31, 2001 Evaluation and Definition of Potentially Hazardous Foods 1. Although each factor affecting growth is considered separately in the following discussion, these factors occur simultaneously in nature. More acidic foods can typically be stored longer without spoiling. â¢The requirements for microbial growth can be divided into two main categories: physical and nutritional. Preserving agents that increase the acidity of food, such as citric acid, are commonly added to help prevent bacterial growth and allow for longer storage. Temperature. Aims: To study the effect of different fermentation conditions and to model the effect of temperature and pH on different biokinetic parameters of bacterial growth and exopolysaccharides (EPS) production of Streptococcus thermophilus ST 111 in milkâbased medium. 2 (Supplement), 2003 âCOMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY 21 Chapter III Factors that Influence Microbial Growth ability to participate in chemical/biochemical reactions, and its availability to facilitate growth of microorganisms. Microbial growth â¢The microbial growth is the increase in number of cells rather than in size of individual cells. Since bacteria are easy to grow in the lab, their growth has been studied extensively. They include: Hydrogen ion concentration (pH), moisture content, nutrient content of the food, antimicrobial substances ad biological structures. Factors affecting Microbial growth Physical factors â¢pH â¢Temperature â¢Osmotic pressure â¢Hydrostatic pressure Factors affecting microbial growth in food (a)Intrinsic factors: These are inherent in the food. Factors that determine whether a particular treatment is -cidal or -static include the types of microorganisms targeted, the concentration of the chemical used, and the nature of the treatment applied. J.N. Methods and Results: The influence of temperature and pH was studied through fermentation and modelling. 9. Growth Curve. As our foods are of plant and animal origin, it is worthwhile to consider those characteristics of plant and animal tissues that affect the growth of microorganisms. Factors affecting microbial growth in food (a) Intrinsic factors: These are inherent in the food. Vinegar and lemon juice have a similar effect. For example, bacteriostatic treatments inhibit the growth of bacteria, whereas fungistatic treatments inhibit the growth of fungi. Introduction The factors discussed in this section constitute an inclusive, rather than exclusive, list of intrinsic, extrinsic, and other factors that may be considered when determining whether a food or Method of Obtaining Bacterial Growth Curve They include: pH, water activity, oxidation reduction potential, nutrient content, antimicrobial contents, biological structure (b) Extrinsic factors: Are factors external to the food that affect microbial growth⦠The acidity of food is also an important factor affecting bacterial growth 1. Inhibition of Microbial Growth. Sofos, in Encyclopedia of Food Safety, 2014. 1. 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